Radboud University Nijmegen Medical Centre; Laboratory for Genetic, Endocrine and Metabolic Disease
With a focus on human specimen, we develop methods for analysis of different types of glycosylation, mainly based on mass spectrometry. In addition, chemically synthesized sugars are applied.
Background
The field of glycobiology has seen an enormous impulse over the last decade with many fascinating features of glycans identified. Groups of the Nijmegen Glycobiology Platform have a long history in studying many aspects of glycan function in human health and disease, resulting in the identification of new glycosylation-related diseases, glycosylation biomarkers for disease, and synthesis of glycopeptides and glycan tracers.
Glycan profiling
MALDI-Ion Trap and nanoChip-QTOF mass spectrometers are currently used for profiling of glycans by molecular mass or for separation of glycan isoforms. For analysis of glycosaminoglycans, disaccharide profiling by HPLC is available.
Glycan tracers
New methodologies for studying glycosylation in cellular systems involve the metabolic labeling of glycoproteins and glycolipids with a variety of synthetic monosaccharides. These can be chemically reacted with biotin probes, after which common biochemical visualization techniques can be applied (western blot, fluorescence, etc.). Tools and more detailed information are available upon request.
Geert Grooteplein 10
6525 GA Nijmegen
The Netherlands
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