GIGA Technology platform - Proteomics

Services:

• Routine analysis:

• Identification of protein Mix (in gel or in solution) by LC-ESI/MS/MS
• High throughput pure protein identification (in gel /in solution) by MALDI-TOF (MS and MSMS)
• Mass determination by MALDI-TOF
• Mass determination by ESI-Q-TOF
• Accurate mass determination by ESI-FTICR
• Top Down sequencing by MALDI-TOF (ISD)
• De novo sequencing by MALDI-TOF-TOF
• Quality Control of protein batch production

• Differential analysis of proteomes:

• 2D-DIGE technology (GE Healthcare): from gels to statistical analysis on Decyder (GE Healthcare)
• Proteins or peptides profiling using SELDI Chips and SELDI-TOF-MS technology (BioRad- PCS4000) for new clinical biomarkers discovery

• Absolute quantification of a known and targeted protein by Liquid Chromatography coupled to isotope dilution mass spectrometry

• In development :

• Post-translational modification analysis by mass spectrometry
• Molecular imaging
• Identification and absolute quantification of the 100 most abundant proteins in a complex mix

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Equipment:

• Bruker ion trap Esquire HCT
• Bruker FT MS APEX-Qe 9.4T
• Bruker MALDI Ultraflex II TOF/TOF
• Micromass (Waters) Q-TOF Ultima global, Quattro Ultima
• Thermo Finnigan LCQ Classic and MAT95’XL
• BioRad - SELDI-TOF (PCS 4000)
• Janus Perkin Elmer workstation for gel spot digestion and processing
• Typhoon TM9400 • Agilent MSDTrap XCT
• Spot picker (GE Healthcare)
• Softwares: Decyder, ImageQuantTL, SameSpot, Image master 2D Platinium
• Mascot server